CURRICULUM VITAERevised 7/31/2013NAME:E-MAIL ADDRESS:Robert Allen [email protected]:1977 B.A.1982 M.S.1989 Ph.D.University of Massachusetts at BostonBoston CollegeBoston CollegeRESEARCH APPOINTMENTS:1983-1988Pre-doctoral Associate, The Jackson Laboratory, Bar Harbor, ME1988-1992Research Fellow in Medicine, Hematology/Oncology, Children's Hospital,Boston, MA1988-1992Research Fellow in Pediatrics, Harvard Medical School, Boston, MA1993Visiting Investigator, Hematology/Oncology, Harvard Medical School andChildren's Hospital, Boston, MA1992-2008Molecular Geneticist, Mouse Genetics Research Laboratory,Children's Mercy Hospitals & Clinics, Kansas City, MO2008-2010DirectorGenetics Research LaboratoryChildren’s Mercy Hospitals & Clinics, Kansas City, MO2010-2012Associate Professor of Molecular Biology/Medical GeneticsDepartment of BiochemistryDybedal Research CenterKansas City University of Medicine & Biosciences, Kansas City, MO2012-presentProfessor of Molecular Biology/Medical Genetics, Department of Biochemistry,Kansas City University of Medicine & Biosciences, Kansas City, MOACADEMIC APPOINTMENTS:1980Lecturer in Genetics, Biology Department, Boston College, Chestnut Hill, MA1981-1983Teaching Fellow, Biology Department, Boston College, Chestnut Hill, MA1988-1992Research Fellow in Pediatrics, Harvard Medical School, Boston, MA
1982-1993Summer Professor, Introductory Biology, Boston College, Chestnut Hill, MA1992-2000Assistant Professor, Department of Pediatrics, University of Missouri-Kansas CitySchool of Medicine, Kansas City, MO2000-2010Associate Professor, Department of Pediatrics, University of Missouri-KansasCity School of Medicine, Kansas City, MO2010-2012Associate Professor of Molecular Biology/Medical Genetics, Department ofBiochemistry, Kansas City University of Medicine & Biosciences, Kansas City,MO2011-presentAdjunct Research Professor of Genetics, University of Missouri-Kansas CitySchool of Medicine, Kansas City, MO2012-presentProfessor of Molecular Biology/Medical Genetics, Department of Biochemistry,Kansas City University of Medicine & Biosciences, Kansas City, MO2013-presentAssociate Dean, College of Biosciences, Kansas City University of Medicine &Biosciences, Kansas City, MOMEMBERSHIPS:American Association for the Advancement of SciencesAmerican Society of HematologyAmerican Society of Human GeneticsInternational Genome SocietyGRANTS:1999-2000Fight for Sight, The Research Division of Prevent Blindness America #GA99047“Gene Targeting of Dp260 Retinal Dystrophin: Analysis of Abnormal ERG andClinical Relevance to Night Blindess” 12,000This funding was used to produce a retinal dystrophin Dp260 knock out mouseand to analyze the effect of specific Dp260 deficiency of the generation of anabnormal ERG.1996-2000The Children’s Mercy Hospital Core Lab Post Doctoral Fellow Grant 140,0002001Paul Patton Memorial Trust Fund“Treatment of Muscular Dystrophy using Novel Gene Expression” 190,000
2000-2003Research Agreement, Menninger Research InstituteA study of the inheritance of the D2 Dopamine Receptor A1 allele and rewarddeficiency syndrome 130,0002001-2004March of Dimes MOD #6-FY012-183“Novel Therapy for Duchenne Muscular Dystrophy: ExpressingRetinal Dystrophin in Muscles of a DMD Mouse” 187,4402001-2005Hall Foundation Grant in Collaboration with Dr. Alan Godwin, KUMC"Isolation of a Novel Gene from a Mouse Model of Human EctodermalDysplasia" 61,0002006-2008U01 HD037249 (P.I. Alexander A. Vinks, Pharm.D., Ph.D.)NICHDPharmacogenetics of Risperidone in Children with Pervasive DevelopmentalDisorder (PDD)Role: DNA genotyping of DRD2 and DRD4 alleles in study patient population 29,1602007-2009KCALSI Research Initiative Award“Transgene Therapy for Cardiomyopathy in DMD Mouse Model” 50,0002011-2012KCALSI Patton Trust Development Grant“Identification of Novel Gene Causing Pediatric Hereditary Spherocytosis” 50,000RESEARCH INTERESTS:The research focus in my lab is the identification of genes involved in the normal production of redblood cells. This has involved using 4 mouse mutants with hereditary anemias. The positional cloning of thecausative genes for these anemia mouse mutants has resulted in their identification and provides theexperimental data for the next phase of this work in molecular biology. The significance of this work is in thepossible development of novel therapies for iron overloading, a condition that leads to severe morbidity anddeath and includes Hereditary Hemochromatosis and transfusion-dependent diseases such as sickle cell anemia.In addition, I have identified the causative gene for a novel model of Hereditary Spherocytosis (HS), the mostcommon hereditary anemia in man with an incidence as high as 1 out of 2,500 in persons of Northern Europeandescent. It is estimated that 10% of patients with HS has no known genetic defect and this discovery may leadto the identification of the defective gene in this significant group. Finally, I am actively pursuing theidentification of a novel erythropoiesis factor in a new mouse mutant with a transient neonatal anemia. Asecond area of research includes developing a gene therapy strategy including the use of a human retinaldystrophin transgene using muscular dystrophy mice.
PUBLICATIONS:MANUSCRIPTS:1. Spark, R. F., Connolly, P. B., White, R. A., Sacks, B. and Landsberg L. (1979) ACTHsecretion from a functioning pheochromocytoma. New England Journal of Medicine 301: 416-418.2. Spark, R. F., White, R. A. and Connolly, P. B. (1980) Impotence is not always psychogenic: newer insightsinto hypothalamic-pituitary-gonadal dysfunction. Journal of the American Medical Association 243:750-755.3. White, R. A. (1988) An inherited erythroid ankyrin deficiency in normoblastosis: a mutation causing severehemolytic anemia. Ph.D. Dissertation. Boston College.4. White, R. A., Birkenmeier, C. S., Lux, S. E. and Barker, J. E. (1990) Ankyrin and the hemolytic anemiamutation, nb, map to mouse Chromosome 8: Presence of the nb allele is associated with a truncatederythroid ankyrin. Proceedings of the National Academy of Sciences USA 83: 3117-3121.5. Peters, L. L., Birkenmeier, C. S., Bronson, R. T., White, R. A., Lux, S. E., Otto, E., Bennett, V., Higgins, A.and Barker, J. E. (1991) Purkinje cell degeneration associated with erythroid ankyrin deficiency innb/nb mice. Journal of Cell Biology 114: 1233-1241.6. Peters, L. L., White, R. A., Birkenmeier, C. S., Lux, S. E. and Barker, J. E. (1992) Changing patterns incytoskeletal mRNA expression and protein synthesis during murine erythropoiesis in vivo. Proceedingsof the National Academy of Sciences USA 89: 5749-5753.7. White, R. A., Birkenmeier, C. S., Peters, L. L., Barker, J. E. and Lux, S. E. (1992) Murine erythrocyteankyrin cDNA: highly conserved regions of the regulatory domain. Mammalian Genome 3: 281-285.8. White, R. A., Peters, L. L., Adkison, L. R., Korsgren, C., Cohen, C. M., and Lux, S. E. (1992) The murinepallid mutation is a platelet storage pool disease associated with the protein 4.2 (pallidin) gene. NatureGenetics 2: 80-83.9. Birkenmeier, C. S., White, R. A., Peters, L. L., Lux, S. E. and Barker, J. E. (1993) Complex patterns ofsequence variation and multiple 5' and 3' ends are found among transcripts of the erythroid ankyrin gene.Journal of Biological Chemistry 268: 9533-9540.10. Adkison, L. R., White, R. A., Haney, D. M., Lee, J. C., Pusey, K. T. and Gardner, J. (1994)The fibronectin receptor, alpha subunit (Itga5) maps to murine Chromosome 15, distal to D15Mit 16.Mammalian Genome 5: 456-457.11. White, R. A., Dowler, L. L., Adkison, L. R., Ezekowitz, R. A. B. and Sastry, K. N. (1994) The murinemannose-binding protein genes (Mbl1 and Mbl2) localize to Chromosomes 14 and 19. MammalianGenome 5: 807-809.
12. White, R. A., Geissler, E. N., Adkison, L. R., Dowler, L. L., Alper, S. L. and Lux, S. E.(1994) Chromosomal location of the murine anion exchanger genes encoding AE2 and AE3.Mammalian Genome 5: 827-829.13. Avraham, S., Jiang, S., Ota, S., Fu, Y., Deng, B., Dowler, L. L., White, R. A. and Avraham,H. (1995) Structural and functional studies of the intracellular tyrosine kinase MATK gene and itstranslated product. Journal of Biological Chemistry 270: 1833-1842.14. White, R. A., Dowler, L. L., Woo, M., Adkison, L. R., Pal, S., Gershon, D. and Fowler, V.M. (1995) The tropomodulin gene (Tmod) maps to Chromosome 4, closely linked to Mup1. MammalianGenome 6: 332-333.15. White, R. A., Dowler, L. L., Pasztor, L. M., Gatson, L. L., Adkison, L. R., Angeloni, S. V.and Wilson, D., B. (1995) Assignment of the transcription factor GATA-4 gene to human chromosome8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization). Genomics 27: 20-26.16. Koeller, D. M., Axtell, K., Angeloni, S. V., Dowler, L. L., Frerman, F. E., White, R. A. andGoodman, S. I. (1995) Cloning, structure and chromosomal localization of the mouse glutaryl-CoAdehydrogenase gene. Genomics 28: 508-512.17. White, R. A., Angeloni, S. V. and Pasztor, L. M. (1995) Chromosomal localization of the βadducin gene to mouse chromosome 6 and human chromosome 2. Mammalian Genome 6: 741-743.18. Hughes, R., Chan, F. Y., White, R. A. and Zon, L. I. (1995) Cloning and chromosomallocalization of a mouse cDNA with homology to the Saccharomyces cerevisiae gene Zuotin. Genomics29: 546-550.19. Adams, M. E., Dwyer, T. M., Dowler, L. L., White, R. A. and Froehner, S. C. (1995) Mouseα1- and β2-syntrophin gene structure, chromosome localization, and homology with a discs-large (PDZ)domain. Journal of Biological Chemistry 270: 25859-25865.20. White, R. A., Dowler, L. L., Angeloni, S. V., Pasztor, L. M. and MacArthur, C. A. (1995)Assignment of FGF8 to human chromosome 10q25-26: mutations in FGF8 may be responsible for sometypes of Acrocephalosyndactyly linked to this region. Genomics 30: 109-111.21. Avraham, S., London, R., Fu, Y., Ota, S., Hiregowdara, D., Pasztor, L. M., White, R. A.,Groopman, J. E. and Avraham, H. (1995) Identification and characterization of a novel related adhesionfocal tyrosine kinase (RAFTK) from megakaryocytes and brain. Journal of Biological Chemistry 270:27742-27751.22. Motwani, M., White, R. A., Guo, N., Dowler, L. L., Tauber, A. I. and Sastry, K. N. (1995)Mouse surfactant protein-D: cDNA cloning, characterization and gene localization to Chromosome 14.Journal of Immunology 155: 5671-5677.
23. White, R. A., Dowler, L. L., Angeloni, S. V., and Koeller, D. M. (1996) Assignment ofEtfdh, Etfb and Etfa to chromosomes 3, 7, and 13: the mouse homologs of genes responsible forglutataric acidemia type II in man. Genomics 33: 131-134.24. White, R. A., Hughes, R. T., Adkison, L. R. and Zon, L. I. (1996) The gene encoding proteinkinase SEK1 maps to mouse chromosome 11 and human Chromosome 17. Genomics 34: 430-432.25. Narita, N., Heikinheimo, M., Bielinska, M., White, R. A., and Wilson, D. B. (1996) Thegene for transcription factor GATA-6 resides on mouse chromosome 18 and is expressed inmyocardium, vascular smooth muscle, and gut epithelium. Genomics 36: 345-348.26. White, R. A., Adkison, L. R., Dowler, L. L., and Ray, R. B. (1997) Chromosomallocalization of the human gene encoding c-myc promoter-binding protein (CMBP1) to Chromosome1p35-pter. Genomics 39: 406-408.27. White, R. A., Dowler, L. L., Hummel, G. S., and Adkison, L. R. (1997) Exclusion of Epb4.2 as a candidatefor the mouse mutant pallid. Mouse Genome 95: 492-494.28. Avraham, S., London, R., Ellis, M., Fu., Y., Jiang, S., White, R. A., Painter, C., andAvaraham, H. (1997) Characterization and chromosomal localization of PTPRO, a novel receptorprotein tyrosine phosphatase, expressed in hematopoietic stem cells. Gene 204: 5-16.29. Brody, S. L., Hackett, B. P., and White, R. A. (1997) Structural characterization of themouse Hfh4 gene, a developmentally regulated forkhead family member. Genomics 45: 509-518.30. Pelletier, G. J., Brody, S. L., Liapis, H., White, R. A., and Hackett, B. P. (1998). A humanforkhead/winged helix transcription factor expressed in developing pulmonary and renal epithelium.Am. J. Physiol. 274: L351-L359.31. McAndrew, P. E., Frostholm, A., White, R. A., Rotter, A., and Burghes, A. H. M. (1998).Identification and characterization of RPTPρ, a novel RPTPμ/κ-like receptor protein tyrosinephosphatase whose expression is restricted to the central nervous system. Mol Brain Res 56: 9-21.32. Lim, B.L., White, R. A., Hummel, G. S., Schwaeble, W., Lynch, N. J., Peerschke, E. I., Reid, K. B., andGhebrehiwet, B. (1998). Characterization of the murine gene of gC1qBP, a novel cell protein that bindsthe globular heada of C1q, vitronectin, high molecular weight kininogen and factor XII. Gene 209:229-237.33. Fitzgerald, K. M., Cibis, G. W., and White, R. A. (1998). Dystrophin localization in cone cells. Invest.Ophthalmol. Vis. Sci. 39: 218-219.34. Jiang, S., Tulloch, A. G., Kim, T.-A., Fu, Y., Rogers, R., Gaskell, A., White, R. A., Avraham, H., andAvraham, S. (1998). Characterization and chromosomal localization of PTP-NP2, a new isoform ofprotein tyrosine phosphatase-like receptor, expressed in brain boutons. Gene 215: 345-359.
35. Dai, G., Chapman, B. M., Liu, B., Orwig, K. E., Wang, D., White, R. A., Preuett, B., and Soares, M. J.(1998). A new member of the mouse prolactin-like protein-C subfamily, prolactin-like protein-Cα:structure and expression. Endocrinology 139: 5157-5163.36. Fitzgerald, K. M., Cibis, G. W., and White, R. A. (1998). ERG in Duchenne/Becker muscular dystrophy.Pediatr. Neurol. 19: 400-401.37. Dai, G., Chapman, B. P., Wang, D., White, R. A., Preuett, B., and Soares, M. J. (1999)Prolactin-like protein-A gene strucuture and chromosomal mapping. Mammalian Genome 10: 78-80.38. Fitzgerald, K. M., Cibis, G. W., Gettel, A. H., Rinaldi, R., Harris, D. J., and WhiteR. A. (1999) ERG phenotype of a dystrophin mutation in heterozygous female carriers of Duchennemuscular dystrophy. J. Med. Genet. 36: 316-322.39. Soltysik-Espanola, M., Rogers, R. A., Jiang, S., Kim, T.-A., G